Collagen Type I anticorps

N° du produit ABIN7566413

L’anticorps Lapin Polyclonal anti-Collagen Type I a été validé pour WB et ICC. Il convient pour détecter Collagen Type I dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Chien, Porc, Mouton, Cat et Hamster.

Aperçu rapide pour Collagen Type I anticorps (ABIN7566413)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Chien, Porc, Mouton, Cat, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Détail du produit anti-Collagen Type I anticorps

Fonction: anti-Collagen Type 1 (3/4 fragment), pAb

Attributs du produit:

Polyclonal Antibody. Recognizes rat and bovine collagen type 1. Based on sequence identity should also recognize human, mouse, guinea pig, dog, cat, donkey, pig, cow, sheep and chicken collagen type 1. Source: Rabbit. Applications: ICC. Liquid. In PBS with 1 mg/mL BSA and 0.02 % sodium azide. The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

Purification: Antigen affinity purified.

Immunogène: Synthetic peptide (human sequence) corresponding to the C-terminal end of the N-terminal three quarter collagen fragment (Col1 3/4).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: In PBS with 1 mg/mL BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type 1