Apelin Receptor anticorps (Extracellular)

N° du produit ABIN7581824

L’anticorps Lapin Polyclonal anti-Apelin Receptor a été validé pour WB, IHC, IF et FACS. Il convient pour détecter Apelin Receptor dans des échantillons de Rat.

Aperçu rapide pour Apelin Receptor anticorps (Extracellular) (ABIN7581824)

Antigène: Apelin Receptor (APLNR)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Apelin Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Apelin Receptor anticorps

Épitope: AA 4-17, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Apelin Receptor

Séquence: DGYNYYGADN QSEC

Specificité: Extracellular, N-terminus

Homologie: Mouse,human - identical

Attributs du produit: Anti-Apelin/APJ Receptor (extracellular) Antibody (ABIN7581824) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Apelin Receptor

Antigène: Apelin Receptor (APLNR)

Autre désignation: APLNR

Sujet: APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34,The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic-pituitary-adrenal (HPA) axis regulation and metabolic homeostasis.Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor's expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92 % homology with mouse receptor and 90 % homology with rat receptor.Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

ID gène: 83518

UniProt: Q9JHG3