Gephyrin anticorps (Intracellular)

N° du produit ABIN7581882

Cet anticorps anti-Gephyrin Polyclonal Cobaye (ABIN7581882) détecte spécifiquement Gephyrin dans WB et IHC. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour Gephyrin anticorps (Intracellular) (ABIN7581882)

Antigène: Gephyrin (GPHN)

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp Gephyrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Gephyrin anticorps

Épitope: AA 183-195, Intracellular

Fonction: A Guinea Pig Polyclonal Antibody to Gephyrin

Séquence: (C)RDAIVKVKEV HDE

Homologie: Mouse,human - identical

Attributs du produit: Guinea Pig Anti-Gephyrin Antibody (ABIN7581882) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gephyrin from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Gephyrin Antibody (ABIN7043214, ABIN7044520 and ABIN7044521) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235588)

Blocking Peptide: (ABIN7235588)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Gephyrin

Antigène: Gephyrin (GPHN)

Autre désignation: GPHN

Sujet:

Synonyms: GPHN, Putative glycine receptor-tubulin linker protein

Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

ID gène: 64845

UniProt: Q03555

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development