G Protein-Coupled Receptor 12 anticorps (Extracellular)

N° du produit ABIN7581884

L’anticorps Lapin Polyclonal anti-G Protein-Coupled Receptor 12 a été validé pour WB et IHC. Il convient pour détecter G Protein-Coupled Receptor 12 dans des échantillons de Souris.

Aperçu rapide pour G Protein-Coupled Receptor 12 anticorps (Extracellular) (ABIN7581884)

Antigène: G Protein-Coupled Receptor 12 (GPR12)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp G Protein-Coupled Receptor 12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-G Protein-Coupled Receptor 12 anticorps

Épitope: AA 3-15, Extracellular

Fonction: A Rabbit Polyclonal antibody to SORCS2

Séquence: (C)EDPKVNLSGL PRD

Specificité: Extracellular, N-term.

Homologie: Rat - identical,Human - 12 out of 13 amino acid residues identical

Attributs du produit: Anti-GPR12 (extracellular) Antibody (ABIN7581884) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12 

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur G Protein-Coupled Receptor 12

Antigène: G Protein-Coupled Receptor 12 (GPR12)

Autre désignation: GPR12 

Sujet: G-Protein Coupled Receptor 12, GPCR21,G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a "constitutively active receptor" since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass. In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.

ID gène: 14738

UniProt: P35412