HCRTR1 anticorps (3rd Intracellular Loop)

N° du produit ABIN7581897

Cet anticorps anti-HCRTR1 Polyclonal Cobaye (ABIN7581897) détecte spécifiquement HCRTR1 dans WB et IHC. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour HCRTR1 anticorps (3rd Intracellular Loop) (ABIN7581897)

Antigène: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp HCRTR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-HCRTR1 anticorps

Épitope: 3rd Intracellular Loop, AA 256-269

Fonction: A Guinea Pig Polyclonal Antibody to Orexin Receptor 1

Séquence: (C)RNWKRPSEQL EAQH

Homologie: Mouse - identical,human - 9 out of 14 amino acid residues identical

Attributs du produit: Guinea Pig Anti-Orexin Receptor 1 Antibody (ABIN7581897) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orexin Receptor 1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235802)

Blocking Peptide: (ABIN7235802)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur HCRTR1

Antigène: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Autre désignation: HCRTR1

Sujet:

Synonyms: OX1R, Hypocretin receptor type 1, HCRTR1

Description: Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

ID gène: 25593

UniProt: P56718

Pathways: Feeding Behaviour