TMEM150C anticorps (Extracellular, Loop 1)

N° du produit ABIN7581992

L’anticorps anti-TMEM150C Polyclonal Lapin est utilisé pour la détection de TMEM150C dans des échantillons de Rat. Il a été validé pour WB, FACS, IHC et LCI.

Aperçu rapide pour TMEM150C anticorps (Extracellular, Loop 1) (ABIN7581992)

Antigène: TMEM150C (Transmembrane Protein 150C (TMEM150C))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TMEM150C est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Détail du produit anti-TMEM150C anticorps

Épitope: AA 33-50, Extracellular, Loop 1

Fonction: A Rabbit Polyclonal antibody to TMEM150C/Tentonin 3 (extracellular).

Séquence: (C)EDDKILPLNS AARKSGVK

Homologie: Mouse - 17 out of 18 amino acid residues identical Human - 16 out of 18 amino acid residues identical

Attributs du produit: Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (ABIN7581992) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM150C from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)EDDKILPLNSAARKSGVK, corresponding to amino acid residues 33 - 50 of rat TMEM150C

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur TMEM150C

Antigène: TMEM150C (Transmembrane Protein 150C (TMEM150C))

Autre désignation: TMEM150C

Sujet:

Synonyms: Transmembrane Protein 150C, TTN3

Description: Transmembrane protein 150C (TMEM150C) also known as Tentonin-3 (TTN3), is a small protein composed of 249 amino acids comprising six transmembrane domains.TMEM150C which was first thought to be a mechanosensitive ion channel that confers a relatively slow inactivating current in proprioceptive neurons in mouse dorsal root ganglion (DRG)1. Later studies, however, showed that the mechanosensitive currents attributed to TMEM150C in different cell setups, could be attributed to the presence of endogenous Piezo1 channels2.Although, some controversy still remains, it is generally accepted that TMEM150C does not form a mechanosensitive channel per se, but rather it functions as a general regulator or sensor, of mechanosensitive channels like Piezo1, Piezo2 and TREK1 (K2P2.1) 2,3.TMEM150C has also been described in the aortic arch and nodose ganglia neurons, where it is involved in the mechanotransduction of arterial pressure to electrical signals in baroreceptors. Dysregulation of TMEM150C alters the sensitivity of the baroreflex, a cardiovascular reflex that is essential for blood pressure homeostasis, leading to diseases such as hypertension, stroke, and heart failure 4.Furthermore, recent evidence suggests that TMEM150C functions as a stretch-activated cation channel in pancreatic β cells, contributing to insulin secretion induced by high glucose levels and hypotonicity. Whether TMEM150C functions in this context as a stretch activated channel itself or whether it functions in conjunction with other proposed stretch-activated channels like TRPV4 or TRPV2, remains to be seen 3,5.

ID gène: 360916

UniProt: B5DFH9