CALHM2 anticorps (Extracellular) (FITC)

N° du produit ABIN7582013

L’anticorps Lapin Polyclonal anti-CALHM2 a été validé pour FACS et LCI. Il convient pour détecter CALHM2 dans des échantillons de Souris.

Aperçu rapide pour CALHM2 anticorps (Extracellular) (FITC) (ABIN7582013)

Antigène: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CALHM2 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-CALHM2 anticorps (FITC)

Épitope: AA 133-146, Extracellular

Fonction: A Rabbit Polyclonal antibody to CALHM2 (extracellular) conjugated to the fluorescent dye FITC

Séquence: (C)SEFVDPSSLT AGDK

Specificité: Extracellular, 2nd loop.

Homologie: Rat - 13 out of 14 amino acid residues identical Human - 11 out of 14 amino acid residues identical

Attributs du produit: Anti-CALHM2 (extracellular) Antibody (ABIN7581840) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples. Anti-CALHM2 (extracellular)-FITC Antibody (ABIN7581840)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide:

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur CALHM2

Antigène: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Autre désignation: CALHM2

Sujet: Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B,Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer's disease.Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4

ID gène: 72691

UniProt: Q8VEC4