GPR4 anticorps (Extracellular) (FITC)

N° du produit ABIN7582035

Cet anticorps anti-GPR4 est un anticorps Lapin Polyclonal détectant GPR4 dans FACS et LCI. Adapté pour Rat.

Aperçu rapide pour GPR4 anticorps (Extracellular) (FITC) (ABIN7582035)

Antigène: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR4 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-GPR4 anticorps (FITC)

Épitope: AA 162-177, Extracellular

Fonction: A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC

Séquence: (C)RDRYNHTF(S)FEKFPME

Specificité: 2nd extracellular loop

Homologie: Rat,mouse,human - 15,16 amino acid residues identical

Attributs du produit: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR4 from mouse, rat and human samples. Anti-GPR4 (extracellular)-FITC Antibody (ABIN7043160, ABIN7044423 and ABIN7044424-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR4 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235415)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR4

Antigène: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Autre désignation: GPR4

Sujet: Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

ID gène: 308408

UniProt: Q4KLH9