NrCAM anticorps (Extracellular) (FITC)

N° du produit ABIN7582053

Cet anticorps anti-NrCAM est un anticorps Lapin Polyclonal détectant NrCAM dans FACS et LCI. Adapté pour Rat.

Aperçu rapide pour NrCAM anticorps (Extracellular) (FITC) (ABIN7582053)

Antigène: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NrCAM est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-NrCAM anticorps (FITC)

Épitope: AA 583-597, Extracellular

Fonction: A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC

Séquence: (C)KDNGELPN DERFSVD

Specificité: Extracellular, N-terminus

Homologie: human - 13,16 amino acid residues identical,Mouse - 15

Attributs du produit: Anti-NrCAM (extracellular) Antibody (ABIN7043369, ABIN7044690 and ABIN7044691) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NrCAM from mouse, rat and human samples. Anti-NrCAM (extracellular)-FITC Antibody (ABIN7043369, ABIN7044690 and ABIN7044691-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NrCAM from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236074)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur NrCAM

Antigène: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Autre désignation: NRCAM

Sujet: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo,NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co-receptor molecule at the neuronal cell surface1,2.NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

ID gène: 497815

UniProt: P97686

Pathways: Regulation of Cell Size