Enolase anticorps

N° du produit ABIN7595318

Cet anticorps Poulet Polyclonal détecte spécifiquement Enolase dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Enolase anticorps (ABIN7595318)

Antigène: Enolase

Reactivité: Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Enolase est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Enolase anticorps

Fonction: Chicken antibody to Enolase

Specificité: Specific for Enolase (alpha beta gamma).

Réactivité croisée: Humain, Marmoset, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: Purified IgY

Immunogène: A synthetic peptide from AA of 10-50 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgY

Information d'application

Indications d'application: WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term.

Date de péremption: 12 months

Détails sur Enolase

Antigène: Enolase

Sujet: Function: Multifunctional enzyme that as well as its role in glycolysis plays a part in various processes such as growth control hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Mammalian enolase is composed of 3 isozyme subunits alpha beta and gamma which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. In vitro interacts with several glycolytic enzymes including PKM2 PGM CKM and aldolase. Also binds troponin in vitro. Subcellular location: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle expression of ENO1 appears to be independent of fiber type.