SARS-CoV-2 Spike anticorps (AA 671-688)

N° du produit ABIN7595334

L’anticorps Cobaye Polyclonal anti-SARS-CoV-2 Spike a été validé pour WB et IHC. Il convient pour détecter SARS-CoV-2 Spike dans des échantillons de SARS Coronavirus-2 (SARS-CoV-2).

Aperçu rapide pour SARS-CoV-2 Spike anticorps (AA 671-688) (ABIN7595334)

Antigène: SARS-CoV-2 Spike

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SARS-CoV-2 Spike est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SARS-CoV-2 Spike anticorps

Épitope: AA 671-688

Fonction: Guinea pig antibody to SARS-Cov-2 spike polybasic region (671-688)

Specificité: Specific for SARS-Cov-2 spike glycoprotein polybasic region

Réactivité croisée: SARS Coronavirus-2 (SARS-CoV-2)

Purification: Whole serum

Immunogène: Synthetic peptide "ASYQTQTNSPRRARSVA" aa 671-688 of SARS spike glycoprotein polybasic region conjugated to blue carrier protein was used as the antigen.

Information d'application

Indications d'application: IHC WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur SARS-CoV-2 Spike

Antigène: SARS-CoV-2 Spike

Sujet: Function: Spike protein S1:attaches the virion to the cell membrane by interacting with host receptor initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. The stalk domain of S contains three hinges giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model the protein has at least three conformational states: pre-fusion native state pre-hairpin intermediate state and post-fusion hairpin state. During viral and target cell membrane fusion the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation thereby counteracting its antiviral activity.