GFP anticorps

N° du produit ABIN7595339

Cet anticorps Carp Polyclonal détecte spécifiquement GFP dans WB, ELISA, IHC et FACS. Il présente une réactivité envers Aequorea victoria.

Aperçu rapide pour GFP anticorps (ABIN7595339)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Carp

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-GFP anticorps

Specificité: Specific for EGFP/GFP/Venus

Réactivité croisée (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgM (mainly)

Immunogène: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgM

Information d'application

Indications d'application: IHC WB ELISA FC. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Use Rabbit anti Carp IgM (H+L) adaptobody at 1:1000 dilution to be able to use your usual anti rabbit secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: EGFP/GFP/Venus

Sujet: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.