eGFP anticorps (AA 1-239)

N° du produit ABIN7596110

Cet anticorps anti-eGFP Monoclonal Souris (Clone AT1D9) (ABIN7596110) détecte spécifiquement eGFP dans WB et ELISA. L’anticorps est réactif avec des échantillons de Aequorea victoria.

Aperçu rapide pour eGFP anticorps (AA 1-239) (ABIN7596110)

Antigène: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp eGFP est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: AT1D9

Détail du produit anti-eGFP anticorps

Épitope: AA 1-239

Fonction: EGFP antibody

Purification: protein-A affinity chromatography

Immunogène: Recombinant EGFP(1-239aa) purified from E. coli

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détails sur eGFP

Antigène: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Autre désignation: EGFP

Sujet: Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.