ANAPC5 anticorps (AA 1-266)

N° du produit ABIN7599186

Cet anticorps anti-ANAPC5 est un anticorps Lapin Polyclonal détectant ANAPC5 dans WB, ELISA, FACS, IF et ICC. Adapté pour Humain.

Aperçu rapide pour ANAPC5 anticorps (AA 1-266) (ABIN7599186)

Antigène: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ANAPC5 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-ANAPC5 anticorps

Épitope: AA 1-266

Fonction: Anti-Apc5/ANAPC5 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Apc5/ANAPC5 Antibody Picoband® (ABIN7599186). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Apc5/ANAPC5 recombinant protein (Position: M1-D266).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. 2. Turnell, A. S., Stewart, G. S., Grand, R. J. A., Rookes, S. M., Martin, A., Yamano, H., Elledge, S. J., Gallimore, P. H. The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression. Nature 438: 690-695, 2005. 3. Yu, H., Peters, J.-M., King, R. W., Page, A. M., Hieter, P., Kirschner, M. W. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279: 1219-1222, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ANAPC5

Antigène: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Autre désignation: ANAPC5

Sujet:

Synonyms: Neuropeptides B/W receptor type 1, G-protein coupled receptor 7, NPBWR1, GPR7

Tissue Specificity: Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea, at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.

Background: Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Poids moléculaire: 85 kDa

ID gène: 51433

Pathways: M Phase