PUM1 anticorps (AA 1-361)

N° du produit ABIN7599311

L’anticorps Lapin Polyclonal anti-PUM1 a été validé pour WB, ELISA, IF, ICC et FACS. Il convient pour détecter PUM1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PUM1 anticorps (AA 1-361) (ABIN7599311)

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-PUM1 anticorps

Épitope: AA 1-361

Fonction: Anti-PUM1 Antibody Picoband®

Attributs du produit: Anti-PUM1 Antibody Picoband® (ABIN7599311). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PUM1 recombinant protein (Position: M1-D361).

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bonnemason-Carrere, P., Morice-Picard, F., Pennamen, P., Arveiler, B., Fergelot, P., Goizet, C., Hellegouarch, M., Lacombe, D., Plaisant, C., Raclet, V., Rooryck, C., Lasseaux, E., Trimouille, A. PADDAS syndrome associated with hair dysplasia caused by a de novo missense variant of PUM1. Am. J. Med. Genet. 179A: 1030-1033, 2019. 2. Gennarino, V. A., Palmer, E. E., McDonell, L. M., Wang, L., Adamski, C. J., Koire, A., See, L., Chen, C.-A., Schaaf, C. P., Rosenfeld, J. A., Panzer, J. A., Moog, U. A mild PUM1 mutation is associated with adult-onset ataxia, whereas haploinsufficiency causes developmental delay and seizures. Cell 172: 924-936, 2018. 3. Gennarino, V. A., Singh, R. K., White, J. J., De Maio, A., Han, K., Kim, J.-Y., Jafar-Nejad, P., di Ronza, A., Kang, H., Sayegh, L. S., Cooper, T. A., Orr, H. T., Sillitoe, R. V., Zoghbi, H. Y. Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type ataxin1 levels. Cell 160: 1087-1098, 2015.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PUM1

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Autre désignation: PUM1

Sujet: Pumilio homolog 1 is a protein that in humans is encoded by the PUM1 gene. This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.

Poids moléculaire: 140 kDa

ID gène: 9698

UniProt: Q14671