TRIM25 anticorps (AA 1-590)

N° du produit ABIN7599491

L’anticorps anti-TRIM25 Polyclonal produit chez le Lapin a été validé pour WB, ELISA, IHC, IF, ICC et FACS. Il convient pour la détection de TRIM25 dans des échantillons provenant de Souris et Rat. }

Aperçu rapide pour TRIM25 anticorps (AA 1-590) (ABIN7599491)

Antigène: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM25 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-TRIM25 anticorps

Épitope: AA 1-590

Fonction: Anti-TRIM25/EFP Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-TRIM25/EFP Antibody (ABIN7599491). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse TRIM25/EFP recombinant protein (Position: M1-H590).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur TRIM25

Antigène: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Autre désignation: Trim25

Sujet:

Synonyms: E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, RING-type E3 ubiquitin transferase, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147

Tissue Specificity: Expressed in breast tumors (at protein level). Ubiquitous.

Background: Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. It is mapped to 17q22. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Poids moléculaire: 71 kDa

ID gène: 217069

UniProt: Q61510