PEPCK anticorps (AA 1-640)

N° du produit ABIN7599509

L’anticorps Souris Monoclonal anti-PEPCK a été validé pour WB, IHC, IF, ICC et FACS. Il convient pour détecter PEPCK dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour PEPCK anticorps (AA 1-640) (ABIN7599509)

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PEPCK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3F7

Détail du produit anti-PEPCK anticorps

Épitope: AA 1-640

Fonction: Anti-PCK2 Antibody Picoband® (monoclonal, 3F7)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PCK2 Antibody Picoband® (monoclonal, 3F7) (ABIN7599509). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PCK2 recombinant protein (Position: M1-M640).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Modaressi, S., Brechtel, K., Christ, B., Jungermann, K. Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene: structure, chromosomal localization and tissue-specific expression Biochem. J. 333: 359-366, 1998. 2. Modaressi, S., Christ, B., Bratke, J., Zahn, S., Heise, T., Jungermann, K. Molecular cloning, sequencing and expression of the cDNA of the mitochondrial form of phosphoenolpyruvate carboxykinase from human liver. Biochem. J. 315: 807-814, 1996.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PEPCK

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Autre désignation: PCK2

Sujet:

Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: Phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2, PEPCK-M), is an isozyme of phosphoenolpyruvate carboxykinase (PCK, PEPCK) that in humans is encoded by the PCK2 gene. It is mapped to 14q11.2-q12. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Poids moléculaire: 71 kDa

ID gène: 5106

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis