POLR2A/RPB1 anticorps (AA 10-321)

N° du produit ABIN7599598

Cet anticorps Lapin Polyclonal détecte spécifiquement POLR2A/RPB1 dans WB, IHC, IF, ELISA, FACS et ICC. Il présente une réactivité envers Humain, Souris, Rat et Singe.

Aperçu rapide pour POLR2A/RPB1 anticorps (AA 10-321) (ABIN7599598)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: AA 10-321

Fonction: Anti-RNA polymerase II/POLR2A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RNA polymerase II/POLR2A Antibody Picoband® (ABIN7599598). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human RNA polymerase II/POLR2A recombinant protein (Position: D10-E321).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Egloff, S., O'Reilly, D., Chapman, R. D., Taylor, A., Tanzhaus, K., Pitts, L., Eick, D., Murphy, S. Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression. Science 318: 1777-1779, 2007. 2. Gnatt, A. L., Cramer, P., Fu, J., Bushnell, D. A., Kornberg, R. D. Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 angstrom resolution. Science 292: 1876-1882, 2001. 3. Haijes, H. A., Koster, M. J. E., Rehmann, H., Li, D., Hakonarson, H., Cappuccio, G., Hancarova, M., Lehalle, D., Reardon, W., Schaefer, G. B., Lehman, A., van de Laar, I. M. B. H., and 32 others. De novo heterozygous POLR2A variants cause a neurodevelopmental syndrome with profound infantile-onset hypotonia. Am. J. Hum. Genet. 105: 283-301, 2019.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A

Sujet:

Synonyms: DNA-ed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-ed RNA polymerase II subunit A, DNA-ed RNA polymerase III largest subunit, RNA-ed RNA polymerase II subunit RPB1, POLR2A, POLR2

Background: DNA-ed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Poids moléculaire: 220 kDa

ID gène: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways