EIF5A anticorps (AA 107-154)

N° du produit ABIN7599674

L’anticorps Lapin Polyclonal anti-EIF5A a été validé pour WB, ELISA, IF, FACS et ICC. Il convient pour détecter EIF5A dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EIF5A anticorps (AA 107-154) (ABIN7599674)

Antigène: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF5A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-EIF5A anticorps

Épitope: AA 107-154

Fonction: Anti-EIF5A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EIF5A Antibody Picoband® (ABIN7599674). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EIF5A recombinant protein (Position: E107-K154).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Doerfel, L. K., Wohlgemuth, I., Kothe, C., Peske, F., Urlaub, H., Rodnina, M. V. EF-P is essential for rapid synthesis of proteins containing consecutive proline residues. Science 339: 85-88, 2013. 2. Faundes, V., Jennings, M. D., Crilly, S., Legraie, S., Withers, S. E., Cuvertino, S., Davies, S. J., Douglas, A. G. L., Fry, A. E., Harrison, V., Amiel, J., Lehalle, D., and 12 others. Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine. Nature Commun. 12: 833, 2021. 3. Gutierrez, E., Shin, B.-S., Woolstenhulme, C. J., Kim, J.-R., Saini, P., Buskirk, A. R., Dever, T. E. eIF5A promotes translation of polyproline motifs. Molec. Cell 51: 35-45, 2013.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur EIF5A

Antigène: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Autre désignation: EIF5A

Sujet:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1

Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Background: Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs. The eIF5A (eIF5A1) and eIF5A2 genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation. Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding AMD1 and eIF5A genes often occur together in human lymphomas.

Poids moléculaire: 18 kDa

ID gène: 1984

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation