EDC4 anticorps (AA 1082-1388)

N° du produit ABIN7599694

Cet anticorps Lapin Polyclonal détecte spécifiquement EDC4 dans ELISA, WB, IF, ICC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour EDC4 anticorps (AA 1082-1388) (ABIN7599694)

Antigène: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EDC4 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-EDC4 anticorps

Épitope: AA 1082-1388

Fonction: Anti-EDC4 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EDC4 Antibody Picoband® (ABIN7599694). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EDC4 recombinant protein (Position: K1082-L1388).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bloch, D. B., Rabkina, D., Quertermous, T., Bloch, K. D. The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. Clin. Immun. Immunopath. 72: 380-389, 1994. 2. Fenger-Gron, M., Fillman, C., Norrild, B., Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Molec. Cell 20: 905-915, 2005. 3. Garcia-Lozano, J. R., Gonzalez-Escribano, M. F., Wichmann, I., Nunez-Roldan, A. Cytoplasmic detection of a novel protein containing a nuclear localization sequence by human autoantibodies. Clin. Exp. Immun. 107: 501-506, 1997.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur EDC4

Antigène: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Autre désignation: EDC4

Sujet:

Synonyms: Protein Dok-7, Downstream of tyrosine kinase 7, DOK7, C4orf25,

Tissue Specificity: Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .

Background: Enhancer of mRNA-decapping protein 4 is a protein that in humans is encoded by the EDC4 gene. Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjgren's syndrome patient later diagnosed with primary biliary cirrhosis. EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation. Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182. Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression.

Poids moléculaire: 152 kDa

ID gène: 23644

UniProt: Q6P2E9