TOP3B anticorps (AA 128-824)

N° du produit ABIN7599907

Cet anticorps anti-TOP3B est un anticorps Lapin Polyclonal détectant TOP3B dans WB, ELISA et IHC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour TOP3B anticorps (AA 128-824) (ABIN7599907)

Antigène: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP3B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-TOP3B anticorps

Épitope: AA 128-824

Fonction: Anti-TOP3B Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-TOP3B Antibody Picoband® (ABIN7599907). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human TOP3B recombinant protein (Position: E128-R824).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kaufman, C. S., Genovese, A., Butler, M. G. Deletion of TOP3B is associated with cognitive impairment and facial dysmorphism. Cytogenet. Genome Res. 150: 106-111, 2016. 2. Kawasaki, K., Minoshima, S., Nakato, E., Shibuya, K., Shintani, A., Schmeits, J. L., Wang, J., Shimizu, N. One-megabase sequence analysis of the human immunoglobulin gamma gene locus. Genome Res. 7: 250-261, 1997. 3. Kwan, K. Y., Moens, P. B., Wang, J. C. Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III-beta. Proc. Nat. Acad. Sci. 100: 2526-2531, 2003.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur TOP3B

Antigène: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Autre désignation: TOP3B

Sujet:

Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: DNA topoisomerase 3-beta-1 is an enzyme that in humans is encoded by the TOP3B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Poids moléculaire: 90-97 kDa

ID gène: 8940

UniProt: O95985