SEC61B anticorps (AA 16-96)

N° du produit ABIN7600188

L’anticorps Lapin Polyclonal anti-SEC61B a été validé pour WB, ELISA et FACS. Il convient pour détecter SEC61B dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SEC61B anticorps (AA 16-96) (ABIN7600188)

Antigène: SEC61B (Sec61 beta Subunit (SEC61B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SEC61B est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-SEC61B anticorps

Épitope: AA 16-96

Fonction: Anti-SEC61B Antibody Picoband®

Attributs du produit: Anti-SEC61B Antibody Picoband® (ABIN7600188). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SEC61B recombinant protein (Position: R16-S96). Human SEC61B shares 98.8% amino acid (aa) sequence identity with mouse SEC61B.

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bebok, Z., Mazzochi, C., King, S. A., Hong, J. S., Sorscher, E. J. The mechanism underlying cystic fibrosis transmembrane conductance regulator transport from the endoplasmic reticulum to the proteasome includes Sec61-beta and a cytosolic, deglycosylated intermediary. J. Biol. Chem. 273: 29873-29878, 1998. 2. Besse, W., Dong, K., Choi, J., Punia, S., Fedeles, S. V., Choi, M., Gallagher, A.-R., Huang, E. B., Gulati, A., Knight, J., Mane, S., Tahvanainen, E., Tahvanainen, P., Sanna-Cherchi, S., Lifton, R. P., Watnick, T., Pei, Y. P., Torres, V. E., Somlo, S. Isolated polycystic liver disease genes define effectors of polycystin-1 function. J. Clin. Invest. 127: 1772-1785, 2017. Note: Erratum: J. Clin. Invest. 127: 3558 only, 2017. 3. Chen, Y., Le Caherec, F., Chuck, S. L. Calnexin and other factors that alter translocation affect the rapid binding of ubiquitin to apoB in the Sec61 complex. J. Biol. Chem. 273: 11887-11894, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur SEC61B

Antigène: SEC61B (Sec61 beta Subunit (SEC61B))

Autre désignation: SEC61B

Sujet: Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

Poids moléculaire: 15 kDa

ID gène: 10952

UniProt: P60468

Pathways: Protein targeting to Nucleus