EIF3E anticorps (AA 160-249)

N° du produit ABIN7600190

Cet anticorps Lapin Polyclonal détecte spécifiquement EIF3E dans WB, ELISA, IHC et FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour EIF3E anticorps (AA 160-249) (ABIN7600190)

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF3E est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-EIF3E anticorps

Épitope: AA 160-249

Fonction: Anti-EIF3E Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EIF3E Antibody Picoband® (ABIN7600190). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EIF3E recombinant protein (Position: A160-M249).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 23477-80. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur EIF3E

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Autre désignation: EIF3E

Sujet:

Synonyms: Eukaryotic translation initiation factor 3 subunit E ,eIF3e ,Eukaryotic translation initiation factor 3 subunit 6 ,Viral integration site protein INT-6 homolog,eIF-3 p48 ,EIF3E ,EIF3S6 , INT6 ,

Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .

Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Poids moléculaire: 52 kDa

ID gène: 3646

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C