Splicing Factor 1 anticorps (AA 160-266)

N° du produit ABIN7600193

Cet anticorps Souris Monoclonal détecte spécifiquement Splicing Factor 1 dans WB, IHC, IF, ICC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Splicing Factor 1 anticorps (AA 160-266) (ABIN7600193)

Antigène: Splicing Factor 1 (SF1)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Splicing Factor 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2F5D10

Détail du produit anti-Splicing Factor 1 anticorps

Épitope: AA 160-266

Fonction: Anti-splicing factor 1 Antibody (monoclonal, 2F5D10)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-splicing factor 1 Antibody (monoclonal, 2F5D10) (ABIN7600193). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Splicing Factor 1

Antigène: Splicing Factor 1 (SF1)

Autre désignation: SF1

Sujet:

Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX

Tissue Specificity: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 68 kDa

ID gène: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location