AGRN anticorps (AA 1864-2068)

N° du produit ABIN7600387

L’anticorps Lapin Polyclonal anti-AGRN a été validé pour IHC, WB, ELISA, ICC, IF et FACS. Il convient pour détecter AGRN dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour AGRN anticorps (AA 1864-2068) (ABIN7600387)

Antigène: AGRN (Agrin (AGRN))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGRN est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-AGRN anticorps

Épitope: AA 1864-2068

Fonction: Anti-Agrin/AGRN Picoband® Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Agrin/AGRN Picoband® Antibody (ABIN7600387). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Agrin/AGRN recombinant protein (Position: S1864-P2068).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bassat, E., Mutlak, Y. E., Genzelinakh, A., Shadrin, I. Y., Umansky, K. B., Yifa, O., Kain, D., Rajchman, D., Leach, J., Bassat, D. R., Udi, Y., Sarig, R., Sagi, I., Martin, J. F., Bursac, N., Cohen, S., Tzahor, E. The extracellular matrix protein agrin promotes heart regeneration in mice. Nature 547: 179-184, 2017. 2. Burgess, R. W., Nguyen, Q.T., Son, Y. J., Lichtman, J. W., Sanes, J. R. Alternatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction. Neuron 23: 33-44, 1999. 3. Campanelli, J. T., Hoch, W., Rupp, F., Kreiner, T., Scheller, R. H. Agrin mediates cell contact-induced acetylcholine receptor clustering. Cell 67: 909-916, 1991.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur AGRN

Antigène: AGRN (Agrin (AGRN))

Autre désignation: AGRN

Sujet:

Synonyms: Agrin, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, C90, Agrin C-terminal 22 kDa fragment, C22, AGRN, AGRIN

Tissue Specificity: Brain specific. Detected in neuronal cells.

Background: Agrin is a protein that in humans is encoded by the AGRN gene. It is mapped to 1p36.33. This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.

Poids moléculaire: 205-217 kDa

ID gène: 375790

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development