PNN anticorps (AA 2-241)

N° du produit ABIN7600477

L’anticorps Lapin Polyclonal anti-PNN a été validé pour WB, ELISA et FACS. Il convient pour détecter PNN dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PNN anticorps (AA 2-241) (ABIN7600477)

Antigène: PNN (Pinin, Desmosome Associated Protein (PNN))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PNN est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-PNN anticorps

Épitope: AA 2-241

Fonction: Anti-PNN/DRSP Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PNN/DRSP Antibody Picoband® (ABIN7600477). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PNN/DRSP recombinant protein (Position: A2-L241).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brandner, J. M., Reidenbach, S., Franke, W. W. Evidence that 'pinin, reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein. Differentiation 62: 119-127, 1997. 2. Brandner, J. M., Reidenbach, S., Kuhn, C., Franke, W. W. Identification and characterization of a novel kind of nuclear protein occurring free in nucleoplasm and in ribonucleoprotein structures of the 'speckle' type. Europ. J. Cell Biol. 75: 295-308, 1998. 3. Degen, W. G. J., Agterbos, M. A., Muyrers, J. P. P., Bloemers, H. P. J., Swart, G. W. M. memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3. Biochim. Biophys. Acta 1444: 384-394, 1999.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PNN

Antigène: PNN (Pinin, Desmosome Associated Protein (PNN))

Autre désignation: PNN

Sujet:

Synonyms: Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A

Tissue Specificity: Detected in peripheral blood monocytes and neutrophils (at protein level).

Background: Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich proteins SRP75 (SRSF4, 601940), SRM300 (SRRM2, 606032), and SRRP130 (PNISR, 616653). Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.

Poids moléculaire: 82 kDa

ID gène: 5411

UniProt: Q9H307