ENDOU anticorps (AA 20-386)

N° du produit ABIN7600552

L’anticorps Lapin Polyclonal anti-ENDOU a été validé pour WB, ELISA, IHC, FACS, ICC et IF. Il convient pour détecter ENDOU dans des échantillons de Humain.

Aperçu rapide pour ENDOU anticorps (AA 20-386) (ABIN7600552)

Antigène: ENDOU (Endonuclease, PolyU-Specific (ENDOU))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ENDOU est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-ENDOU anticorps

Épitope: AA 20-386

Fonction: Anti-PP11/ENDOU Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PP11/ENDOU Antibody Picoband® (ABIN7600552). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PP11/ENDOU recombinant protein (Position: K20-T386).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 2/24/2014. 2. Grundmann, U., Romisch, J., Siebold, B., Bohn, H., Amann, E. Cloning and expression of a cDNA encoding human placental protein 11, a putative serine protease with diagnostic significance as a tumor marker. DNA Cell Biol. 9: 243-250, 1990. 3. Laneve, P., Gioia, U., Ragno, R., Altieri, F., Di Franco, C., Santini, T., Arceci, M., Bozzoni, I., Caffarelli, E. The tumor marker human placental protein 11 is an endoribonuclease. J. Biol. Chem. 283: 34712-34719, 2008.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur ENDOU

Antigène: ENDOU (Endonuclease, PolyU-Specific (ENDOU))

Autre désignation: ENDOU

Sujet:

Synonyms: Mesoderm posterior protein 1, Class C basic helix-loop-helix protein 5, bHLHc5, MESP1, BHLHC5

Tissue Specificity: Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

Background: Endonuclease, poly(U) specific is a protein that in humans is encoded by the ENDOU gene. This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

Poids moléculaire: 47 kDa

ID gène: 8909

UniProt: P21128