PRAME anticorps (AA 21-509)

N° du produit ABIN7600624

L’anticorps Lapin Polyclonal anti-PRAME a été validé pour WB et ELISA. Il convient pour détecter PRAME dans des échantillons de Humain.

Aperçu rapide pour PRAME anticorps (AA 21-509) (ABIN7600624)

Antigène: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRAME est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-PRAME anticorps

Épitope: AA 21-509

Fonction: Anti-PRAME Picoband® Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PRAME Picoband® Antibody (ABIN7600624). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PRAME recombinant protein (Position: T21-N509).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Epping, M. T., Wang, L., Edel, M. J., Carlee, L., Hernandez, M., Bernards, R. The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling. Cell 122: 835-847, 2005. 2. Ikeda, H., Lethe, B., Lehmann, F., van Baren, N., Baurain, J.-F., de Smet, C., Chambost, H., Vitale, M., Moretta, A., Boon, T., Coulie, P. G. Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor. Immunity 6: 199-208, 1997. 3. van Baren, N., Chambost, H., Ferrant, A., Michaux, L., Ikeda, H., Millard, I., Olive, D., Boon, T., Coulie, P. G. PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells. Brit. J. Haemat. 102: 1376-1379, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PRAME

Antigène: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Autre désignation: PRAME

Sujet:

Synonyms: Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, OIP-4, Preferentially expressed antigen of melanoma, PRAME, MAPE

Tissue Specificity: Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.

Background: Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene. It is mapped to 22q11.22. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 58 kDa

ID gène: 23532

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding