U2AF2 anticorps (AA 238-470)

N° du produit ABIN7600793

Cet anticorps anti-U2AF2 est un anticorps Lapin Polyclonal détectant U2AF2 dans WB, ELISA, IF, IHC, ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour U2AF2 anticorps (AA 238-470) (ABIN7600793)

Antigène: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp U2AF2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-U2AF2 anticorps

Épitope: AA 238-470

Fonction: Anti-U2AF65/U2AF2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-U2AF65/U2AF2 Antibody Picoband® (ABIN7600793). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human U2AF65/U2AF2 recombinant protein (Position: M238-H470).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kielkopf, C. L., Rodionova, N. A., Green, M. R., Burley, S. K. A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer. Cell 106: 595-605, 2001. 2. Mackereth, C. D., Madl, T., Bonnal, S., Simon, B., Zanier, K., Gasch, A., Rybin, V., Valcarcel, J., Sattler, M. Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature 475: 408-411, 2011. 3. Sickmier, E. A., Frato, K. E., Shen, H., Paranawithana, S. R., Green, M. R., Kielkopf, C. L. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Molec. Cell 23: 49-59, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur U2AF2

Antigène: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Autre désignation: U2AF2

Sujet:

Synonyms: Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, hU2AF (65), Hu2af65, U2 snRNP auxiliary factor large subunit, U2AF2, U2AF65

Tissue Specificity: Ubiquitous.

Background: Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Poids moléculaire: 65 kDa

ID gène: 11338

UniProt: P26368