Liver Arginase anticorps (AA 25-183)

N° du produit ABIN7600888

L’anticorps Souris Monoclonal anti-Liver Arginase a été validé pour WB et FACS. Il convient pour détecter Liver Arginase dans des échantillons de Humain, Rat, Souris et Singe.

Aperçu rapide pour Liver Arginase anticorps (AA 25-183) (ABIN7600888)

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivité: Humain, Rat, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Liver Arginase est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 2B12

Détail du produit anti-Liver Arginase anticorps

Épitope: AA 25-183

Fonction: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (ABIN7600888). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3μ/1x10⁶ cells, Human1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. Mutat. 14: 355-356, 1999. 2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic study of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989. 3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and nucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Liver Arginase

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Autre désignation: ARG1

Sujet:

Synonyms: Putative Polycomb group protein ASXL1, Additional sex combs-like protein 1, ASXL1, KIAA0978

Tissue Specificity: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes.

Background: ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98 % of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90 % of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50 % of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 35 kDa

ID gène: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin