EVL anticorps (AA 268-400)

N° du produit ABIN7600986

Cet anticorps Lapin Polyclonal détecte spécifiquement EVL dans WB, ELISA, IHC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour EVL anticorps (AA 268-400) (ABIN7600986)

Antigène: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EVL est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-EVL anticorps

Épitope: AA 268-400

Fonction: Anti-EVL Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EVL Antibody Picoband® (ABIN7600986). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EVL recombinant protein (Position: M268-E400).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 4/19/2016. 2. Janssens, K., De Kimpe, L., Balsamo, M., Vandoninck, S., Vandenheede, J. R., Gertler, F., Van Lint, J. Characterization of EVL-I as a protein kinase D substrate. Cell. Signal. 21: 282-292, 2009. 3. Klostermann, A., Lutz, B., Gertler, F., Behl, C. The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A-1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein-like protein (EVL) via a novel carboxyl-terminal zyxin-like domain. J. Biol. Chem. 275: 39647-39653, 2000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur EVL

Antigène: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Autre désignation: EVL

Sujet:

Synonyms: Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252, Tnfsf4, Ox40l, Txgp1l

Tissue Specificity: Macrophages, peripheral blood leukocytes, lung, spleen and liver.

Background: Ena/VASP-like protein is a member of the Ena/VASP family of proteins that in humans is encoded by the EVL gene. Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells. The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization. Research studies have shown that EVL also regulates DNA repair by interaction with RAD51. EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination. Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer.

Poids moléculaire: 50 kDa

ID gène: 51466