Claudin 18 anticorps (AA 31-261)

N° du produit ABIN7601257

L’anticorps Lapin Polyclonal anti-Claudin 18 a été validé pour WB, IHC, ELISA, FACS, ICC et IF. Il convient pour détecter Claudin 18 dans des échantillons de Humain et Rat.

Aperçu rapide pour Claudin 18 anticorps (AA 31-261) (ABIN7601257)

Antigène: Claudin 18 (CLDN18)

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Claudin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-Claudin 18 anticorps

Épitope: AA 31-261

Fonction: Anti-Claudin18/CLDN18 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Claudin18/CLDN18 Antibody Picoband® (ABIN7601257). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Claudin18/CLDN18 recombinant protein (Position: S31-V261).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Niimi, T., Nagashima, K., Ward, J. M., Minoo, P., Zimonjic, D. B., Popescu, N. C., Kimura, S. claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and stomach-specific isoforms through alternative splicing. Molec. Cell. Biol. 21: 7380-7390, 2001.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Claudin 18

Antigène: Claudin 18 (CLDN18)

Autre désignation: CLDN18

Sujet:

Synonyms: Tudor domain-containing protein 3, TDRD3

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Claudin-18 is a protein that in humans is encoded by the CLDN18 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Poids moléculaire: 30 kDa

ID gène: 51208

UniProt: P56856

Pathways: Cell-Cell Junction Organization, Hepatitis C