NEFL anticorps (AA 4-463)

N° du produit ABIN7601626

L’anticorps Lapin Polyclonal anti-NEFL a été validé pour WB, IHC, IF, ELISA et FACS. Il convient pour détecter NEFL dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour NEFL anticorps (AA 4-463) (ABIN7601626)

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEFL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Détail du produit anti-NEFL anticorps

Épitope: AA 4-463

Fonction: Anti-NEFL/NF-L Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-NEFL/NF-L Antibody Picoband® (ABIN7601626). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human NEFL/NF-L recombinant protein (Position: F4-A463).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Berciano, J., Garcia, A., Peeters, K., Gallardo, E., De Vriendt, E., Palayo-Negro, A. L., Infante, J., Jordanova, A. NEFL E396K mutation is associated with a novel dominant intermediate Charcot-Marie-Tooth disease phenotype. J. Neurol. 262: 1289-1300, 2015. 2. Hurst, J., Flavell, D., Julien, J.-P., Meijer, D., Mushynski, W., Grosveld, F. The human neurofilament gene (NEFL) is located on the short arm of chromosome 8. Cytogenet. Cell Genet. 45: 30-32, 1987. 3. Leung, C. L., Nagan, N., Graham, T. H., Liem, R. K. H. A novel duplication/insertion mutation of NEFL in a patient with Charcot-Marie-Tooth disease. Am. J. Med. Genet. 140A: 1021-1025, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur NEFL

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Autre désignation: NEFL

Sujet:

Synonyms: Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP, Squalene- and sterol-carrier protein, SCP, Z-protein, p14, Fabp1

Tissue Specificity: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Background: Neurofilament light polypeptide (NFL), also known as neurofilament light chain, is a neurofilament protein that in humans is encoded by the NEFL gene. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Poids moléculaire: 72 kDa

ID gène: 4747

UniProt: P07196