HNRNPK anticorps (AA 40-463)

N° du produit ABIN7601650

L’anticorps Lapin Polyclonal anti-HNRNPK a été validé pour WB, ELISA, IF, ICC et FACS. Il convient pour détecter HNRNPK dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour HNRNPK anticorps (AA 40-463) (ABIN7601650)

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HNRNPK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-HNRNPK anticorps

Épitope: AA 40-463

Fonction: Anti-hnRNP K/HNRNPK Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-hnRNP K/HNRNPK Antibody Picoband® (ABIN7601650). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human hnRNP K/HNRNPK recombinant protein (Position: D40-F463).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Au, P. Y. B., Goedhart, C., Ferguson, M., Breckpot, J., Devriendt, K., Wierenga, K., Fanning, E., Grange, D. K., Graham, G. E., Galarreta, C., Jones, M. C., Kini, U., Stewart, H., Parboosingh, J. S., Kline, A. D., Innes, A. M., Care for Rare Canada Consortium. Phenotypic spectrum of Au-Kline syndrome: a report of six new cases and review of the literature. Europ. J. Hum. Genet. 26: 1272-1281, 2018. 2. Au, P. Y. B., You, J., Caluseriu, O., Schwartzentruber, J., Majewski, J., Bernier, F. P., Ferguson, M., Care for Rare Canada Consortium, Valle, D., Parboosingh, J. S., Sobreira, N., Innes, A. M., Kline, A. D. GeneMatcher aids in the identification of a new malformation syndrome with intellectual disability, unique facial dysmorphisms, and skeletal and connective tissue abnormalities caused by de novo variants in HNRNPK. Hum. Mutat. 36: 1009-1014, 2015. 3. Dejgaard, K., Leffers, H., Rasmussen, H. H., Madsen, P., Kruse, T. A., Gesser, B., Nielsen, H., Celis, J. E. Identification, molecular cloning, expression and chromosome mapping of a family of transformation upregulated hnRNP-K proteins derived by alternative splicing. J. Molec. Biol. 236: 33-48, 1994.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur HNRNPK

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Autre désignation: HNRNPK

Sujet:

Synonyms: Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2, RUNX1T1, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2

Tissue Specificity: Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.

Background: Heterogeneous nuclear ribonucleoprotein K (also protein K) is a protein that in humans is encoded by the HNRNPK gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference, it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.

Poids moléculaire: 60 kDa

ID gène: 3190

UniProt: P61978