NNT anticorps (AA 44-350)

N° du produit ABIN7601742

Cet anticorps Lapin Polyclonal détecte spécifiquement NNT dans WB, ELISA, IF, ICC et FACS. Il présente une réactivité envers Humain, Souris, Rat, Porc et Singe.

Aperçu rapide pour NNT anticorps (AA 44-350) (ABIN7601742)

Antigène: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Reactivité: Humain, Souris, Rat, Porc, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NNT est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-NNT anticorps

Épitope: AA 44-350

Fonction: Anti-NNT Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-NNT Antibody Picoband® (ABIN7601742). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human NNT recombinant protein (Position: C44-A350).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat, Pig
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hasselmann, C., Deladoey, J., Vuissoz, J.-M., Patry, L., Alirezaie, N., Schwartzentruber, J., FORGE Canada Consortium, Deal, C. L., Van Vliet, G., Majewski, J., Samuels, M. E. Expanding the phenotypic spectrum of nicotinamide nucleotide transhydrogenase (NNT) mutations and using whole exome sequencing to discover potential disease modifiers. J. Genomes Exomes 2: 19-30, 2013. 2. Meimaridou, E., Kowalczyk, J., Guasti, L., Hughes, C. R., Wagner, F., Frommolt, P., Nurnberg, P., Mann, N. P., Banerjee, R., Saka, H. N., Chapple, J. P., King, P. J., Clark, A. J. L., Metherell, L. A. Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency. Nature Genet. 44: 740-742, 2012. 3. Roucher-Boulez, F., Mallet-Motak, D., Samara-Boustani, D., Jilani, H., Ladjouze, A., Souchon, P.-F., Simon, D., Nivot, S., Heinrichs, C., Ronze, M., Bertagna, X., Groisne, L., Leheup, B., Naud-Saudreau, C., Blondin, G., Lefevre, C., Lemarchand, L., Morel, Y. NNT mutations: a cause of primary adrenal insufficiency, oxidative stress and extra-adrenal defects. Europ. J. Endocr. 175: 73-84, 2016.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur NNT

Antigène: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Autre désignation: NNT

Sujet:

Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK

Tissue Specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.

Background: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD (H) and NADP (+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Poids moléculaire: 114 kDa

ID gène: 23530

UniProt: Q13423

Pathways: Proton Transport