Occludin anticorps (AA 54-270)

N° du produit ABIN7601995

L’anticorps anti-Occludin Polyclonal Lapin est utilisé pour la détection de Occludin dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA, FACS, IF et ICC.

Aperçu rapide pour Occludin anticorps (AA 54-270) (ABIN7601995)

Antigène: Occludin (OCLN)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Occludin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Occludin anticorps

Épitope: AA 54-270

Fonction: Anti-Occludin/OCLN Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Occludin/OCLN Antibody (ABIN7601995). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Occludin/OCLN recombinant protein (Position: H54-K270).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: OCLN

Sujet:

Synonyms: Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2, CDKN1C, KIP2

Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

Background: Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Poids moléculaire: 65 kDa

ID gène: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C