Ribonuclease H1 anticorps (AA 7-223)

N° du produit ABIN7602317

Cet anticorps anti-Ribonuclease H1 est un anticorps Lapin Polyclonal détectant Ribonuclease H1 dans WB, ELISA, ICC, IF et FACS. Adapté pour Humain et Souris.

Aperçu rapide pour Ribonuclease H1 anticorps (AA 7-223) (ABIN7602317)

Antigène: Ribonuclease H1 (RNASEH1)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ribonuclease H1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Ribonuclease H1 anticorps

Épitope: AA 7-223

Fonction: Anti-RNASEH1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RNASEH1 Antibody Picoband® (ABIN7602317). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human RNASEH1 recombinant protein (Position: L7-Q223).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cerritelli, S. M., Crouch, R. J. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase H1. Genomics 53: 300-307, 1998. 2. Cerritelli, S. M., Frolova, E. G., Feng, C., Grinberg, A., Love, P. E., Crouch, R. J. Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. Molec. Cell 11: 807-815, 2003. 3. Nowotny, M., Gaidamakov, S. A., Ghirlando, R., Cerritelli, S. M., Crouch, R. J., Yang, W. Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Molec. Cell 28: 264-276, 2007. Note: Erratum: Molec. Cell 28: 513 only, 2007.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Ribonuclease H1

Antigène: Ribonuclease H1 (RNASEH1)

Autre désignation: RNASEH1

Sujet:

Synonyms: Spindle and kinetochore-associated protein 2, Protein FAM33A, SKA2, FAM33A

Background: Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene. This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are ed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.

Poids moléculaire: 38 kDa

ID gène: 246243

UniProt: O60930