WAPAL anticorps (AA 916-1190)

N° du produit ABIN7602663

Cet anticorps Lapin Polyclonal détecte spécifiquement WAPAL dans ELISA, WB, IF, IHC, ICC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour WAPAL anticorps (AA 916-1190) (ABIN7602663)

Antigène: WAPAL (Wings Apart-Like Protein Homolog (WAPAL))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WAPAL est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-WAPAL anticorps

Épitope: AA 916-1190

Fonction: Anti-WAPL/FOE Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-WAPL/FOE Antibody Picoband® (ABIN7602663). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human WAPL/FOE recombinant protein (Position: Q916-C1190).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Busslinger, G. A., Stocsits, R. R., van der Lelij, P., Axelsson, E., Tedeschi, A., Galjart, N., Peters, J.-M. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature 544: 503-507, 2017. 2. Hill, L., Ebert, A., Jaritz, M., Wutz, G., Nagasaka, K., Tagoh, H., Kostanova-Poliakova, D., Schindler, K., Sun, Q., Bonelt, P., Fischer, M., Peters, J.-M., Busslinger, M. Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature 584: 142-147, 2020. 3. Kueng, S., Hegemann, B., Peters, B. H., Lipp, J. J., Schleiffer, A., Mechtler, K., Peters, J.-M. Wapl controls the dynamic association of cohesin with chromatin. Cell 127: 955-967, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur WAPAL

Antigène: WAPAL (Wings Apart-Like Protein Homolog (WAPAL))

Autre désignation: WAPL

Sujet:

Synonyms: p-selectin glycoprotein ligand, Selplg, Psgl1

Tissue Specificity: Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach.

Background: WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth. It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by ly interacting with its regulatory subunits. WAPL is a new regulator of the development and metastasis of cancerous tissue.

Poids moléculaire: 180 kDa

ID gène: 23063

UniProt: Q7Z5K2

Pathways: Chromatin Binding