PDIA3 anticorps (C-Term)

N° du produit ABIN7602778

L’anticorps Souris Monoclonal anti-PDIA3 a été validé pour WB, IHC et FACS. Il convient pour détecter PDIA3 dans des échantillons de Humain.

Aperçu rapide pour PDIA3 anticorps (C-Term) (ABIN7602778)

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDIA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 7E5

Détail du produit anti-PDIA3 anticorps

Épitope: C-Term

Fonction: Anti-ERp57 Antibody Picoband® (monoclonal, 7E5)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ERp57 Antibody Picoband® (monoclonal, 7E5) (ABIN7602778). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human ERp57, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: PDIA3 protein disulfide isomerase family A, member 3". 2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006 3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PDIA3

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Autre désignation: PDIA3

Sujet:

Synonyms: Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, K8, Type-II keratin Kb8, KRT8, CYK8

Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Background: PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase, however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Poids moléculaire: 57 kDa

ID gène: 2923

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis