Claudin 16 anticorps (C-Term)

N° du produit ABIN7602819

Cet anticorps Lapin Polyclonal détecte spécifiquement Claudin 16 dans WB, FACS, ICC et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Claudin 16 anticorps (C-Term) (ABIN7602819)

Antigène: Claudin 16 (CLDN16)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Claudin 16 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-Claudin 16 anticorps

Épitope: C-Term

Fonction: Anti-CLDN16/Claudin-16 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CLDN16/Claudin-16 Antibody Picoband® (ABIN7602819). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin CLDN16/Claudin-16, which shares 95% and 95% amino acid (aa) sequence identity with mouse and rat Claudin CLDN16/Claudin-16, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bardet, C., Courson, F., Wu, Y., Khaddam, M., Salmon, B., Ribes, S., Thumfart, J., Yamaguti, P. M., Rochefort, G. Y., Figueres, M.-L., Breiderhoff, T., Garcia-Castano, A., and 27 others. Claudin-16 deficiency impairs tight junction function in ameloblasts, leading to abnormal enamel formation. J. Bone Miner. Res. 31: 498-513, 2016. 2. Kausalya, P. J., Amasheh, S., Gunzel, D., Wurps, H., Muller, D., Fromm, M., Hunziker, W. Disease-associated mutations affect intracellular traffic and paracellular Mg2+ transport function of claudin-16. J. Clin. Invest. 116: 878-891, 2006. 3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Claudin 16

Antigène: Claudin 16 (CLDN16)

Autre désignation: CLDN16

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: Claudin-16 is a protein that in humans is encoded by the CLDN16 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28.

Poids moléculaire: 34 kDa

ID gène: 10686

UniProt: Q9Y5I7

Pathways: Hepatitis C