CENPB anticorps (C-Term)

N° du produit ABIN7602867

Cet anticorps Lapin Polyclonal détecte spécifiquement CENPB dans WB, IF, ICC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour CENPB anticorps (C-Term) (ABIN7602867)

Antigène: CENPB (Centromere Protein B (CENPB))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CENPB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-CENPB anticorps

Épitope: C-Term

Fonction: Anti-CENPB Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CENPB Antibody Picoband® (ABIN7602867). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human CENPB, identical to the related mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Cam, H. P., Noma, K., Ebina, H., Levin, H. L., Grewal, S. I. S. Host genome surveillance for retrotransposons by transposon-derived proteins. Nature 451: 431-436, 2008. 2. Earnshaw, W. C., Sullivan, K. F., Machlin, P. S., Cooke, C. A., Kaiser, D. A., Pollard, T. D., Rothfield, N. F., Cleveland, D. W. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. J. Cell Biol. 104: 817-829, 1987. 3. Fowler, K. J., Hudson, D. F., Salamonsen, L. A., Edmondson, S. R., Earle, E., Sibson, M. C., Choo, K. H. A. Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. Genome Res. 10: 30-41, 2000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur CENPB

Antigène: CENPB (Centromere Protein B (CENPB))

Autre désignation: CENPB

Sujet:

Synonyms: Sodium- and chloride-dependent GABA transporter 1,GAT-1,Solute carrier family 6 member 1,SLC6A1,GABATR, GABT1, GAT1,

Tissue Specificity: Expressed in many tissues, highest levels in skeletal muscle.

Background: Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. In humans, centromere protein B is encoded by the CENPB gene. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Poids moléculaire: 80 kDa

ID gène: 1059

UniProt: P07199