Cathepsin L anticorps (Middle Region)

N° du produit ABIN7603032

Cet anticorps Lapin Polyclonal détecte spécifiquement Cathepsin L dans WB, IHC et FACS. Il présente une réactivité envers Souris et Rat.

Aperçu rapide pour Cathepsin L anticorps (Middle Region) (ABIN7603032)

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cathepsin L est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-Cathepsin L anticorps

Épitope: Middle Region

Fonction: Anti-Cathepsin L/MEP/Ctsl Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Cathepsin L/MEP/Ctsl Antibody Picoband® (ABIN7603032). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of mouse Cathepsin L/MEP/Ctsl, which shares 70% and 95% amino acid (aa) sequence identity with human CTSL, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Abudula, A., Rommerskirch, W., Weber, E., Gunther, D., Wiederanders, B. Splice variants of human cathepsin L mRNA show different expression rates. Biol. Chem. 382: 1583-1591, 2001. 2. Arora, S., Chauhan, S. S. Identification and characterization of a novel human cathepsin L splice variant. Gene 293: 123-131, 2002. 3. Bakhshi, R., Goel, A., Seth, P., Chhikara, P., Chauhan, S. S. Cloning and characterization of human cathepsin L promoter. Gene 275: 93-101, 2001.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Cathepsin L

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Autre désignation: Ctsl

Sujet:

Synonyms: Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A

Tissue Specificity: Detected in peripheral blood monocytes and neutrophils (at protein level).

Background: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.

Poids moléculaire: 20-42 kDa

ID gène: 13039

UniProt: P06797

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades