TFAM anticorps (N-Term)

N° du produit ABIN7603113

Cet anticorps Souris Monoclonal détecte spécifiquement TFAM dans WB, IHC et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour TFAM anticorps (N-Term) (ABIN7603113)

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TFAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 4D9

Détail du produit anti-TFAM anticorps

Épitope: N-Term

Fonction: Anti-mtTFA TFAM Antibody (monoclonal, 4D9)

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: different from the related mouse,rat sequences by five amino acids.

Attributs du produit: Anti-mtTFA TFAM Antibody.(monoclonal, 4D9)-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 1-2 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur TFAM

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Autre désignation: TFAM

Sujet:

Background: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Gene Full Name: transcription factor A, mitochondrial

Poids moléculaire: 40 kDa

ID gène: 7019

UniProt: Q00059

Pathways: Chromatin Binding