YTHDF2 anticorps (N-Term)

N° du produit ABIN7603191

Cet anticorps Lapin Polyclonal détecte spécifiquement YTHDF2 dans WB, IHC, IF, FACS et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour YTHDF2 anticorps (N-Term) (ABIN7603191)

Antigène: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp YTHDF2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-YTHDF2 anticorps

Épitope: N-Term

Fonction: Anti-YTHDF2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-YTHDF2 Antibody Picoband® (ABIN7603191). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human YTHDF2, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Bonafe, M., Cardelli, M., Marchegiani, F., Cavallone, L., Giovagnetti, S., Olivieri, F., Lisa, R., Pieri, C., Franceschi, C. Increase of homozygosity in centenarians revealed by a new inter-Alu PCR technique. Exp. Gerontol. 36: 1063-1073, 2001. 2. Cardelli, M., Marchegiani, F., Cavallone, L., Olivieri, F., Giovagnetti, S., Mugianesi, E., Moresi, R., Lisa, R., Franceschi C. A polymorphism of the YTHDF2 gene (1p35) located in an Alu-rich genomic domain is associated with human longevity. J. Gerontol. A Biol. Sci. Med. Sci. 61: 547-556, 2006. 3. Liu, N., Dai, Q., Zheng, G., He, C., Parisien, M., Pan, T. N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions. Nature 518: 560-564, 2015.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur YTHDF2

Antigène: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Autre désignation: YTHDF2

Sujet:

Synonyms: Homeobox protein SIX3,Sine oculis homeobox homolog 3,SIX3,

Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Background: YTH N6-methyladenosine RNA binding protein 2 is a protein that in humans is encoded by the YTHDF2 gene. This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Poids moléculaire: 65 kDa

ID gène: 51441

UniProt: Q9Y5A9