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GFP anticorps (AA 25-120) (FITC)

L’anticorps Lapin Polyclonal anti-GFP a été validé pour WB, IF (p) et IF (cc). Il convient pour détecter GFP dans des échantillons de Aequorea victoria et Aequorea coerulescens (Belt Jellyfish).
N° du produit ABIN729440

Aperçu rapide pour GFP anticorps (AA 25-120) (FITC) (ABIN729440)

Antigène

Voir toutes GFP Anticorps
GFP (Green Fluorescent Protein (GFP))

Reactivité

  • 180
  • 14
  • 11
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)

Hôte

  • 75
  • 63
  • 34
  • 14
  • 9
  • 5
  • 5
  • 4
  • 1
Lapin

Clonalité

  • 114
  • 92
Polyclonal

Conjugué

  • 91
  • 13
  • 9
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  • 4
  • 4
  • 3
  • 3
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Cet anticorp GFP est conjugé à/à la FITC

Application

  • 151
  • 74
  • 57
  • 49
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Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
  • Épitope

    • 16
    • 14
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    • 4
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    AA 25-120

     Réactivité croisée

    Porc, Tag

    Réactivité croisée (Details)

    Aequorea victoria, Aequorea coerulescens (belt jellyfish)

    Purification

    Purified by Protein A.

    Immunogène

    KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

    Isotype

    IgG
  • Indications d'application

    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Agent conservateur

    ProClin

    Précaution d'utilisation

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    GFP (Green Fluorescent Protein (GFP))

    Autre désignation

    GFP

    Sujet

    Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.

    Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

    UniProt

    P42212
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