GFP anticorps (AA 25-120) (HRP)

N° du produit ABIN729442

Cet anticorps Lapin Polyclonal détecte spécifiquement GFP dans WB, ELISA, IHC (fro) et IHC (p). Il présente une réactivité envers Aequorea victoria et Aequorea coerulescens (Belt Jellyfish).

Aperçu rapide pour GFP anticorps (AA 25-120) (HRP) (ABIN729442)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GFP anticorps (HRP)

Épitope: AA 25-120

Réactivité croisée: Porc, Tag

Réactivité croisée (Details): Aequorea victoria, Aequorea coerulescens (belt jellyfish)

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet:

Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt: P42212