Liver Arginase anticorps (AA 11-97)

N° du produit ABIN7649679

Cet anticorps anti-Liver Arginase est un anticorps Souris Monoclonal détectant Liver Arginase dans WB, IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour Liver Arginase anticorps (AA 11-97) (ABIN7649679)

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Liver Arginase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ARG1-1125

Détail du produit anti-Liver Arginase anticorps

Épitope: AA 11-97

Fonction: Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125)

Attributs du produit: This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human ARG1 protein fragment (around aa11-97)

Isotype: IgG3, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: 293Tcells. Hepatocellular Carcinoma (HCC).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Liver Arginase

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Autre désignation: Arginase 1

Sujet:

Synonyms: Arginase 1, ARG1, liver-type arginase, type I arginase

Gene Symbol: ARG1

Tissue Expression: Liver

Poids moléculaire: 35-38 kDa

ID gène: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin