Recombinant Biotin anticorps (CF®647)

N° du produit ABIN7650066

Cet anticorps anti-Biotin Monoclonal Lapin conjugué à CF®647 (Clone BTN-2032R) (ABIN7650066) détecte spécifiquement Biotin dans IHC et IHC (fp). L’anticorps est réactif avec des échantillons de Différentes espèces.

Aperçu rapide pour Recombinant Biotin anticorps (CF®647) (ABIN7650066)

Antigène: Biotin

Type d'anticorp: Recombinant Antibody

Reactivité: Différentes espèces

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BTN-2032R

Détail du produit anti-Biotin anticorps (CF®647)

Fonction: Biotin (Vitamin B7 or Vitamin H) (BTN/2032R), CF647 conjugate

Attributs du produit: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Biotinylated sheep immunoglobulin

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. In situ Nucleic Acid Hybridization. No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.. Western blotting. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Biotinylated proteins in solution or on tissues

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet:

Synonyms: Vitamin B7 or Vitamin H

Gene Symbol: Not Applicable

Poids moléculaire: 244Da