Recombinant CNN1 anticorps (CF®740)

N° du produit ABIN7650460

Cet anticorps Lapin Monoclonal détecte spécifiquement CNN1 dans FACS, IF, IHC et IHC (fp). Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour Recombinant CNN1 anticorps (CF®740) (ABIN7650460)

Antigène: CNN1 (Calponin 1 (CNN1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CNN1 est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CNN1-1408R

Détail du produit anti-CNN1 anticorps (CF®740)

Fonction: Calponin-1 (Smooth Muscle Marker) (CNN1/1408R), CF740 conjugate

Attributs du produit: Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length CNN1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Myoepithelial cells in breast ducts

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CNN1

Antigène: CNN1 (Calponin 1 (CNN1))

Autre désignation: Calponin-1

Sujet:

Synonyms: Calponin-1, CNN1, Calponin 1 basic smooth muscle, Calponin H1 smooth muscle

Gene Symbol: CNN1

Tissue Expression: Smooth muscle

Poids moléculaire: 34 kDa

ID gène: 1264

UniProt: P51911