CEA anticorps (CF®740)

N° du produit ABIN7650564

Cet anticorps anti-CEA est un anticorps Souris Monoclonal détectant CEA dans IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour CEA anticorps (CF®740) (ABIN7650564)

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CEA est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: COL-1 CEA31 C66-261

Détail du produit anti-CEA anticorps (CF®740)

Fonction: CD66 (CEA)(COL-1 + CEA31 + C66/261), CF740 conjugate

Attributs du produit: This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA positive) from pleural mesotheliomas (rarely or weakly CEA positive). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human colon carcinoma extract (COL-1 & CEA31); Recombinant full-length human CEA protein (C66/261)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF7 or 293T cells. Colon carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CEA

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Autre désignation: Carcinoembryonic Antigen

Sujet:

Synonyms: Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

Gene Symbol: CEACAM1 & CEACAM5

Poids moléculaire: 80-200 kDa

ID gène: 1048, 634

UniProt: P06731